Stroud Group

Research

Mitochondria are the main source of energy in eukaryotic cells, oxidizing sugars and fats to generate ATP through oxidative phosphorylation (OXPHOS), which is accomplished by the respiratory chain (Figure 1). Defects in OXPHOS affect the respiratory chain and lead to classical childhood mitochondrial disease, which has an incidence of at least ~1/5000 live births. Organs with the highest energy demand such as brain and heart are normally affected, although the disease can affect any organ system alone or in combination. Mutations in almost 200 genes encoding mitochondrial proteins involved in all aspects of OXPHOS cause mitochondrial disease.

Diagnostic approaches typically focus on targeted sequencing of known mitochondrial disease genes, whereas the genetic complexity of the disease means drugs targeting one OXPHOS system often fail when another is defective.

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Our research aims to functionalise the mitochondrial proteome, and couple this with the development of unbiased quantitative proteomics approaches to assist in diagnosis and inform future treatment strategies.

Figure 1: OXPHOS and the mitochondrial respiratory chain

Techniques

We employ cutting-edge systems biology approaches, incorporating extensive gene-editing and quantitative proteomics tools, as well as classical biochemical, molecular and cell biology techniques.

Follow this link to publications for David Stroud compiled by Google Scholar.

Group Members

Group Head: David Stroud

Postdoctoral Fellow: Joanna Sacharz

Research Assistant: Daniella Hock

Biography

David Stroud is an NHMRC Career Development Fellow and laboratory head within the Department of Biochemistry and Molecular Biology, University of Melbourne. He has an interest in the development and application of new technologies to better understand the assembly of multi-protein membrane complexes, with a focus on mitochondria. David was awarded his doctorate from the University of Freiburg, Germany in 2011 under the supervision of Prof. Klaus Pfanner. In 2012 David returned to Australia and was awarded an NHMRC Peter Doherty ECR Fellowship. Since then, his work has been focused on mitochondrial respiratory chain assembly and miss-assembly in disease. Under the mentorship of Prof. Mike Ryan, David established a mitochondrial systems biology research program which moved to The University of Melbourne in 2018. David’s work combines traditional biochemistry and molecular biology techniques with an in-house proteomics pipeline, and he routinely employs technologies such as SILAC labelling, whole cell proteomes and affinity enrichment mass-spectrometry (including proximity tagging approaches such BioID) to answer basic biological questions.