- Platform Technologies
Mass Spectrometry and Proteomics
The Mass Spectrometry and Proteomics Facility provides academic and industry researchers access to specialist mass spectrometry and proteomic equipment and expertise.
The facility is Open Access.
We teach you to perform your own mass spectrometry and proteomics experiments using our instrumentation.
All advice on the planning, execution, and analysis of your samples is included in the cost. We have taught people to:
- Process their own gel spots,
- Characterize intact recombinant proteins by mass,
- Confirm a chemical synthesis,
- Identify phosphorylation sites,
- Immune precipitation of protein complexes, and
- Perform both labeled and label-free quantitation experiments.
We have more than 300 users from more than 70 different labs all over Victoria and Interstate as evidence that this approach is very effective. We have a fleet of eight mass spectrometers and we make it as easy as possible for you to learn as much or as little as you like about the actual instrumentation.
Our costs are based on a membership structure. The facility access cost is highly subsidized by the University of Melbourne and is charged on a quarterly, or yearly basis. A laboratory head will pay the subscription cost ($5100 for University of Melbourne) and then every laboratory member can access the platform.
Like a gym membership you can run as many samples as you like during your subscription.
The only limit is that as a shared facility you cannot monopolize or run samples in preference to anyone else. The greatest benefit of using our facility is that you learn how to do it yourself. We frequently find that users start very simple but as they get more experience they branch out to ever more complex and imaginative uses for the technology. They start to redesign how they do things in order to take advantage of new workflows and more precise answers – why spend hours on an SDS-PAGE gel for a recombinant protein when in 15 minutes you can confirm its primary structure with an EXACT mass on the full length protein?
At present we have:
- 2 x Thermo NanoLC/ OrbiTrap Fusion Lumos
- 2 x Thermo NanoLC/ Q Exactive Plus Mass spectrometer
- Thermo NanoLC/ OrbiTRAP ELITE ETD mass spectrometer
- Themo Exactive Focus
- 2 x Agilent 6220 LC/ESI-TOF mass spectrometer
- ABSCIEX 5500 NanoLC/QTRAP Mass spectrometer
- Bruker Microflex MALDI-TOF Mass spectrometer
- Shimadzu UHPLC 8060 QQQ
The laboratory also has a number of general equipment items for use:
- Deadbox for proteomic sample preparation
- Labconco SpeediVac system
- Vertis benchtop freeze dryer
We also have:
- Four Agilent HPLC systems for sample preparation.
They are also open access instruments.
Access to the Mass Spectrometers
To access the mass spectrometers contact:
Dr Nicholas Williamson
nawill [at] unimelb.edu.au (Dr Nicholas Williamson)
Manager of the Mass Spectrometry and Proteomics Facility
Tel: (03) 8344 2206)
Tel: (03) 8344 2540
Users will only be granted access after they have attended a formal training session.