Mass Spectrometry and HPLC ‘101’ Lecture Series
Mass spectrometry has emerged as a core analytical technique for chemistry and medical research sciences. These five lectures will provide a basic working knowledge of mass spectrometry and HPLC. The later lectures will address proteomics and metabolomics. This series of lectures is deliberately aimed at new MS users with explanations kept as simple as possible and with an emphasis placed on understanding the results.
Free event. Attend one, or attend all five lectures. All welcome.
When: Monday 21 to Friday 25 September 2015
Time: 2pm to 4pm each day in the Bio21 Auditorium
Where: Bio21 Institute Auditorium, 30 Flemington Rd, Parkville
Lectures presented by
Dr Nicholas Williamson: Director, Bio21 Mass Spectrometry and Proteomics Facility
Dr Berin Boughton: Research Fellow, Metabolomics Australia, School of Biosciences
Dr David Perkins: Senior Bioinformatician, Bio21 Mass Spectrometry and Proteomics Facility
Mr Chris Bowen: Research Fellow, Metabolomics Australia, Bio21
Lecture 1 HPLC 101
• How does it separate things?
• Buffer compatibility
• Troubleshooting problems
• Fittings and tubing
Lecture 2 General Mass Spec concepts
• Isotope and charge series
• Buffer compatibility
• Electrospray
• MALDI
• Charge deconvolution
Lecture 3 Proteomic Mass Spec
• Sample preparation
• MS\MS sequencing
• Mass estimates on recombinant proteins
• testing synthetic peptides,
• how to do gel spots
Lecture 4 Metabolomics and Mass Spec
• What is GCMS?
• Aminio acids, sugar, Lipids
Lecture 5 Proteomic bioinformatics
• What is MASCOT – how does it work?
• Ingenuity for proteomic network analysis
Activity: Interested in how to run a gel spot? Email Nick Williamson to register for this half day hands‐on session in the lab on Wednesday and Thursday. Maximum 8 people. Email: nawill [at] unimelb.edu.au
