Magnetic Resonance

Magnetic Resonance spectroscopy determines the structures of molecules ranging from small chemicals to macromolecular proteins and nucleic acids. It is particularly useful for the analysis of proteins that cannot be crystallised and for investigating interactions between proteins, biological membranes and ligands, including potential new drugs. The Bio21 Institute's substantial Magnetic Resonance facility is one of Australia's leading Magnetic Resonance centres.

At the Bio21 Institute, Magnetic Resonance is primarily used for investigating structures of potential drug and pesticide targets, protein pharmaceuticals and include metabolite biomarkers of disease as well as interactions of proteins with their specific ligands (including candidates drugs) and, where relevant, their target membranes. Magnetic Resonance capability is also important to a range of analytical and synthetic chemistry applications.

The critical mass developed by bringing together this capability makes the Bio21 Institute's Magnetic Resonance facility a valuable resource for attracting industry and academic researchers. The facility's Magnetic Resonance spectrometers are all available on a fee-for-use basis. They include:

Spectrometers:

400 MHz

  • Agilent MR400 and autosampler suited to small molecules where sensitivity is not a dominant issue.
  • Bruker Avance IIIHD wide-bore 3-channel system with 500W/300W RF amplifiers and x{H} and X{H/F} MAS probes

500 MHz

  • Agilent DD2 system equipped with an autosampler and a broadband probe suited to 13C, 31P, 19F and 77Se. Suited to small molecules where sensitivity is not a dominant issue.
  • Bruker Avance system equipped with TXI cryoprobe for greater proton sensitivity and suitable for peptides and small proteins.

600 MHz

  • A Bruker Avance III system with TCI cryoprobe and BACS-60 autosampler. Suited to moderate size proteins and molecules where sensitivity begins to be an issue.
  • A Varian INOVA 600 equipped with a broadband probe suited to small molecules where senstivity is not an issue.
  • Varian VNMRS narrow-bore 4-channel HFXY system with kWatt 1H and 13C RF amplifiers and a suite of MAS and static probes for bio-solid studies including phospholipid membranes.

700 MHz

  • Bruker Avance IIIHD with TCI cryoprobe and SampleJet autosampler for high-throughput applications such as metabolomics.

800 MHz

  • Bruker Avance II with TCI cryoprobe and BACS-60 autosampler. Suited to large proteins where high sensitivity and resolution are required and for metabolomic studies requiring high throughput.

Accessing the Magnetic Resonance Spectrometers

Please contact granth [at] unimelb.edu.au (Hamish Grant) for more information.

All new staff members and students who wish to access the Magnetic Resonance Facility must complete an induction. Please download and study the induction document before attending the induction presentation at a date to be advised.

Group Members

Dr David Keizer

Dr Hamish Grant

Dr Shenggen Yao

Paul O'Donnell