- Platform Technologies
“Viruses pose one of the grand challenges to human and animal health globally and within Australia. The overarching aim of our research is to understand the mechanisms by which viruses cause disease, and so identify novel targets and innovative strategies to develop new vaccines and therapeutics for currently incurable viral diseases.” - Greg Moseley
Research in the Moseley laboratory seeks to elucidate the molecular interactions formed by viruses with the infected cell, and to understand their roles in diseases caused highly lethal human viruses including rhabdoviruses (e.g. rabies virus, Australian bat lyssavirus), paramyxoviruses (e.g. Nipah, Hendra, measles) and filoviruses (e.g. Ebola), as well as a number of agriculturally significant and potentially zoonotic animal viruses. The overarching aim of the research is to identify novel targets and strategies for the development of new vaccines and therapeutics against currently incurable viral diseases.
The research projects involve extensive collaborations within the University of Melbourne, and with other leading national (e.g. CSIRO-Australian Animal Health Lab., Monash University and MIMR, University of Sydney) and international institutes (e.g. The Pasteur Institute and CNRS, Paris; Gifu and Hokkaido Universities, Japan; DUKE-NUS; Mt Sinai Med. School, New York), enabling access to unique resources and technologies including novel and highly pathogenic viruses.
- Characterisation of the immune evasion mechanisms of novel and emerging viruses
- Viral interactions with the cytoskeleton in the manipulation of host cell biology
- Virus-STAT interactions: roles in disease and therapeutic targeting
- The roles of intranuclear viral protein interactions in disease
Molecular cell biology, molecular virology, and molecular immunology approaches, including dynamic live cell imaging (quantitative confocal laser scanning microscopy, fluorescence recovery after photobleaching [FRAP], Förster resonance energy transfer/fluorescence-lifetime imaging microscopy [FRET/FLIM], Raster image correlation spectroscopy, bimolecular fluorescence complementation), super-resolution light microscopy (including direct stochastic optical reconstruction microscopy [dSTORM]) and electron microscopy, in vivo microtubule interaction assays (including quantitative cytoskeletal imaging), functional genomics and proteomics (including next-generation sequencing, RT-PCR, mass-spectroscopy), viral reverse genetics and infection, in vivo viral infection/disease models, immune cytokine signalling assays (including reporter gene and RT-PCR assays), in vitro protein trafficking/interaction assays, cell culture and transfection (including stable and inducible cell lines), siRNA knockdown, flow cytometry, immunoprecipitation, recombinant protein expression and purification.
Kim Gia Lieu
Graduate Students and Research Assistants
Greg Moseley is head of the Viral Pathogenesis Laboratory. After graduating with BSc(Hons) from the University of York, UK, he undertook research toward a PhD at The University of Sheffield in the UK and the Walter and Eliza Hall Institute on the roles of tetraspanin proteins in immunology and infection. Greg was subsequently awarded a fellowship from the Royal Society which enabled him to undertake postdoctoral research in immunology at the Austin Research Institute (Melbourne). He later moved to Monash University to pursue research investigating the mechanisms of subcellular protein trafficking. At Monash University he established an independent research laboratory bringing together his research expertise in virology, protein trafficking and immunology to investigate the molecular mechanisms underlying viral immune evasion and pathogenicity. Greg also undertook fellowship research in CNRS (France) and Gifu University (Japan). In 2013 Greg was awarded the Grimwade Fellowship and relocated his laboratory to the Department of Biochemistry and Molecular Biology at the Bio21 Institute.